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Ecl solution western blot8/8/2023 This ECL kit provides a comparable level of sensitivity to Supersignal™ West Femto (Thermo Fisher Scientific) Amersham™ ECL Select (GE Healthcare) with the same antibody dilutions and protocol, and does not require protocol re-optimization or changes from these kits. Contents include the following: GERPN2109, 62. Mix ECL Plus Solution A and ECL Plus Solution. Compared to entry-substrates, SignalFire ECL Reagent boasts a more robust signal and extended duration of signal output. Successively probe western blots with anti-p42/44 MAPK (to detect total MAPK proteins), antiphospho-p42/44. ECL Extreme has a chemiluminescence intensity 150 times greater than standard ECL substrates and gives bright, clear bands at super-low protein concentrations. ECL Western Blotting Detection Reagents in combination with Hybond ECL membranes form a complete solution for Western blot applications. SignalFire ECL Reagent from Cell Signaling Technology (CST) is an enhanced chemiluminescent substrate capable of detecting picogram amounts of protein by western blot analysis. The solution is sensitive enough to detect approximately 50fg of protein per band and has a signal duration of up to 8 hours. It uses an enhanced chemiluminescent substrate for western blotting that was developed for film imaging and is also compatible with CCD imaging. , Size SignalFire ECL Reagent is sensitive enough to detect picogram levels of protein. 3.2.1 Mix an equal volume of EZ-ECL Solution A and EZ-ECL Solution B to give sufficient. LumiBlue ECL Extreme is a western blotting substrate with extreme signal intensity and is most effective for very dilute, low abundance applications where maximum sensitivity is essential. Product overview High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band. Protocol for Western Blotting and Chemiluminescence Detection. Enhanced Chemiluminescence (ECL) Extreme solution for Western blotting
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